*Seminar Series: IMB Chemistry and Structural Biology Division Seminar
*Location: Seminar Room 3.142, QBP Bldg 80, UQ St Lucia
*Date: 21/09/2017
*Time: 12:30

Speaker #1 details
*Title of talk: Cloning and characterization of LIC1, a ligand-gated ion channel from the algae Chlamydomonas reinhardtii

*Speaker's name: Dr. Argel Estrada
 (Lynch Lab)
*Speaker's organisation: Queensland Brain Institute
, University of Queensland
Speaker's city/state/country:
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Talk Abstract: Pentameric Ligand-Gated Ion Channels are key elements for the generation of action potentials in the brain of vertebrates, but little is known about the molecular evolution of these proteins. One member of this family is present in the unicellular algae Chlamydomonas reinhardtii, a model organism for further research. Chlamydomonas elicits action potentials which are related to their motility, here we characterize some electrophysiological and phenotypical properties of this channel by means of a knock-out strain, and hypothesize about its possible role in the biology of the algae, as well as its potential as an ancestral protein previous to the development of the synapse along the evolution.
Speaker's bio: Dr. Estrada is a postdoc in the Lynch lab at The Queensland Brain Institute. Recently he has collaborated with the Hankamer group on pentameric Ligand-Gated Ion Channels using molecular and biophysical approaches.

Speaker #2 details
*Title of talk: Electron tomography: High throughput cell segmentation
*Speaker's name: Dr. Rubbiya Ali (Hankamer Lab)
*Speaker's organisation: Institute for Molecular Bioscience, The University of Queensland
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Talk Abstract: The visualization of 3D cellular ultrastructure at atomic resolution is a major goal of cellular and structural biology. High-throughput molecular segmentation of cells to the level of organelles, macromolecular assemblies and membrane proteins in membranes is a critical component of this process, to facilitate sub-volume extraction, molecular docking and meta-data annotation. We developed a new 3D image filter, RAZA (Rapid, Automated 3D z-crossings Algorithm) to semi-automate cell segmentation. RAZA was able to extract edge details defining the contours of organelles, macromolecular assemblies and membrane proteins.
Speaker's bio: Dr. Rubbiya Ali is a post-doctoral research fellow in the Hankamer lab. She is working on data analysis, management and visualisation of 2D/3D electron microscopy data, and has developed novel 3D methods related to edge detection, segmentation, and particle picking.

Other Details:
*Host name: Professor David Fairlie
Host phone number:
*Host email: d.fairlie@imb.uq.edu.au