*Seminar Series: IMB Special Cell Biology Seminar
*Location: Large Seminar Room (3.142), Level 3 Qld Bioscience Precinct Building 80, St Lucia
*Date: 17/08/2017
*Time: 14:00

Speaker #1 details
*Title of talk: Lectin-driven and glycosphingolipid-dependent construction of endocytic pits
*Speaker's name: Prof. Ludger Johannes
*Speaker's organisation: Curie Institute
Speaker's city/state/country: Paris, France
Website:
Talk Abstract: Several endocytic processes do not require the activity of clathrin, and it has been a major question in membrane biology to know how the plasma membrane is bent and cargo proteins are sorted in these cases. Our previous studies have allowed us to propose a novel hypothesis, termed GL-Lect hypothesis: nanodomain construction by GlycosphingoLipid-binding cellular or pathological Lectins induces membrane curvature changes and drives the formation of endocytic pits for the cellular uptake of glycosylated membrane proteins with critical roles in cell migration (CD44, alpha5beta1 integrin…), of pathogens (polyoma viruses, norovirus) or pathogenic factors (Shiga and cholera toxins). We are now analyzing how cortical actin dynamics contributes to the clustering of glycosphingolipid-lectin complexes on active membranes, thereby facilitating the
nucleation of endocytic tubules exploiting fluctuation forces that had not been linked before to endocytosis. Furthermore, we are identifying mechanisms by which the GL-Lect mechanism is acutely controlled at the plasma membrane. Finally, we study how GL-Lect domain construction at the plasma membrane programs the intracellular distribution of cargo molecules, notably via the retrograde transport route.
Speaker's bio: Ludger Johannes (PhD) is Research Director (DR1) at INSERM.

Since the beginning of his biochemistry undergraduate studies in 1987, he is member of the Studienstiftung des Deutschen Volkes (German organization of the academically gifted), and since 1993 of Boehringer Ingelheim Fonds. Between 2001 and 2013, he directed the Traffic, Signaling and Delivery Group in the Cell Biology Department (UMR144 CNRS) of Institut Curie. Since
January 2014, he is heading the Chemical Biology of Membranes and Therapeutic Delivery unit (U1143 INSERM — UMR3666 CNRS). His research aims at establishing fundamental concepts of endocytosis and intracellular trafficking. The Johannes group has made two major contributions in this context: the discovery of a membrane trafficking interface between early endosomes and the Golgi apparatus, and the demonstration that dynamic protein-induced glycosphingolipid reorganization acts as a driving force for membrane invagination in clathrin-independent endocytosis. These studies are well cited and have been published in several high-ranking journals, including Cell, Nature, Nature Cell Biology, Developmental Cell, and The Journal of Cell Biology. He also aims at exploiting these discoveries in fundamental membrane biology research for the development of innovative cancer therapy strategies. His basic studies have allowed him to validate the B-subunit of Shiga toxin (STxB)
as a "pilot" for the delivery of therapeutic compounds to precise intracellular locations of dendritic and tumor cells (10 patent families, 5 of which are delivered in the US, Europe and
other countries). These findings are the basis for a translational research program on intracellular delivery at the Curie Institute, and for the creation of biotech companies. Ludger Johannes serves on the INSERM study section on cell and developmental biology, on editorial boards of several international journals (including PLoS One and Traffic), and is EMBO member since 2012. His group is member of LabEx CelTisPhysBio, and he currently holds an ERC senior grant (2014-2019).

Speaker #2 details
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Other Details:
*Host name: Prof Rob Parton
Host phone number:
*Host email: r.parton@imb.uq.edu.au