*Seminar Series: SCMB Chemistry Seminar
*Location: AIBN Seminar Room
*Date: 03/07/2017
*Time: 13:00

Speaker #1 details
*Title of talk: Development of Novel Sensitive Methods for Biologics and Vaccine Formulations
*Speaker's name: A/Prof. Veysel Kayser
*Speaker's organisation: University of Sydney
Speaker's city/state/country: NSW
Website: http://sydney.edu.au/pharmacy/about/people/profiles/veysel.kayser.php
Talk Abstract: Many biologics and vaccines suffer from formulation stability issues due to their complex nature and also because of complicated manufacturing steps. Generally, it is difficult to elucidate molecular interactions in these products. For example, the degradation of monoclonal antibodies (mABs) occurs mainly via aggregation and is poorly understood. Aggregates are known to be immunogenic, therefore understanding its mechanism and accurate quantification is important. Several methods to address this issue and to facilitate the development of mAbs will be presented.
Another example is flu vaccines which are produced by chemical inactivation and surfactant treatment of the viruses. Surfactant causes viruses to 'split' by solubilising membrane and further stabilizes proteins. This 'splitting' process affects the formulation stability and potency of flu vaccines greatly. Consequently, finding the ideal splitting conditions, being able to estimate the split ratio, and quantifying aggregates and residual surfactant in each step is of utmost importance for rapid preparation of flu vaccines. I will show some of our recent findings from a collaboration with industry.
A third example is engineered peptide systems where self-assembly mechanism and/or monomer-aggregate transition is of interest in order to develop stable peptide-based products.
I will present several approaches - employing spectroscopy, chromatography and microscopy, to:
* Detect a stable mAb from a library and protein aggregation in mAb formulations.
* Estimate the split ratio of flu virus following surfactant treatment and determine protein aggregates in influenza vaccines.
* Evaluate molecular interactions and elucidate self-assembling mechanism in engineered peptides.
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Speaker #2 details
*Title of talk:
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Other Details:
*Host name: Elizabeth Krenske
Host phone number:
*Host email: e.krenske@uq.edu.au