*Seminar Series: Chemistry and Structural Biology Division Seminar
*Location: IMB Seminar Room 3.142
*Date: 08/06/2017
*Time: 12:30

Speaker #1 details
*Title of talk: Intracellular Screening for Antagonists of Proteins
*Speaker's name: Dr Jody Mason
*Speaker's organisation: Department of Biosciences, University of Bath
Speaker's city/state/country: UK
Talk Abstract: My research focuses on design of peptide antagonists with high affinity and specificity for inhibiting protein-protein interactions. While forces driving stability are now well understood, much less is known about designing specificity. We use a Protein-fragment Complementation Assay (PCA), which uses semi-rational design to select antagonists of Jun and Fos peptides that interact in the oncogenic Activator Protein-1 transcriptional regulator. We have devised a Competitive and Negative Design Initiative (CANDI) technique to increase target-specificity in the PCA system by expressing potential off-targets during selection. Peptides generated allow us to predict coiled coil stability and specificity based only on the primary sequence. Our screening approach is also being applied to a range of other therapeutically relevant systems that include β-amyloid and α-synuclein implicated in the pathogenesis of Alzheimer's and Parkinson's diseases, respectively.
Speaker's bio: Jody graduated from the University of Bristol, U.K. in 1997. In 2001, he completed a PhD in protein folding at the Department of Biochemistry, University of Bristol under the supervision of the late Professor Tony Clarke. Postdoctoral research at the University of Manchester focussed on amyloid aggregation inhibitors with Professor Andrew Doig. He then moved to the University of Freiburg, Germany before taking up a Lectureship at the University of Essex in 2007. Since 2015 Jody is a Senior Lecturer at the University of Bath focussed on peptide-library screening and design of stable and specific protein-protein interactions. Jody is a member of the BBSRC pool of experts and the Alzheimer's Research UK Grant Review Board.

Speaker #2 details
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Other Details:
*Host name: Professor David Fairlie
Host phone number:
*Host email: d.fairlie@imb.uq.edu.au